Project Outline:

''Effects of hydrogen sulphide on autophagy: a tissue culture study''
Over the next 8 weeks I'll be culturing HeLa cells and exposing them to up and down regulation of intracellular hydroden sulphide levels, using drug tre
atments, siRNAs and plasmids coding for its generative enzymes CBS an
d CSE. We will then ascertain hydrogen sulphides effects on autophagy (if any), primarily by confocal fluorescence microscopy, gel electrophoresis and western blot analysis.

Thursday 5 July 2012

A fortnight of science!

Science; Slippery when wet
So it's been an eventfull couple of weeks as the first half of my placement draws to a close! The heavens opened over Newcastle causing mass floods and creating a wash-out in ground floor the lobby of our lab. But science continued on! Us being on the second floor and all.

In other news researchers in China published a paper on my project area last week, and have observed a decrease in levels of autophagy as hydrogen sulphide levels increase. Preliminary data from my western blots and fluorescence assays seem to confirm their findings, so we'll continue on and see what else we can find out.

Definition: i·ro·ny/ˈīrənē/

. An ageing research laboratory overlooking a graveyard.


I'm about to write up all the data I've so far collected to present at my second lab meeting tomorrow. Hopefully there's some good numbers we can infer from and get a feel for what the enzymes and chemicals Im assaying for are doing inside our HeLa cells!

The good news is I'm finally getting to grips with all the protocols I'm expected to do. I'm screwing less things up each day and with any luck next week, nothing will go wrong =D and we can look forward to some nice reliable data.

Here's a nice diagram from my first presentation outlining what Im doing!


More to report tomorrow after all my results are in!